Ghent Light Microscopy CORE

Wide-field microscopes

Overview of wide field microscopes present at the expertise centre and there unique features:

 Nikon Ti widefield fluorescence microscope (inverted)

Instrument specificationseclipseti.png

  • Filter sets (UV to red)

    • DIA/DIC ANALYSER

    • DAPI (Ex. 387/11, Em. 447/60)

    • GFP-B (Ex. 472/30, Em. 520/35)

    • TRITC (Ex. 543/22, Em. 593/40)

    • Cy5 (Ex. 628/40, Em. 692/40)

    • GFP-L (472/30, Em. 488LP)

  • Light sensitive, cooled EM-CCD camera (1000*1000 pixels)
  • Nikon colour CMOS camera
  • Incubator with temperature regulation at the microscope level and CO2 at the cell culture level
  • Fully automatic filter wheels, objectives, XY-Stage, light source, ...
  • Perfect Focus System
  • Extended software for recording and visualization of multi-dimensional (X, Y, Z, Lambda, t) images

Contact us for more information

Location

Faculty Bioscience Engineering,

Vakgroep Biotechnologie, BW25

Campus Proeftuin, Gebouw N1, Lokaal 36.01.100.030

B-9000, Ghent

Price

Price category 1

Leica DM6 B: automated upright fluorescence microscope.

Instrument specifications

  • Moterized z-focus and stage
  • Stage insert for 4 slides
  • White light LED 
  • Metal halide lamp (fluorescence)
  • Filter sets
    • DAPI(Ex. BP 360/40, DM LM400, Em. BP470/40)
    • BLUE (Ex. BP 480/40, DM 505, Em. BP527/30)
    • RHOD (Ex. BP 546/10, DM LP560, Em.  BP585/40)
    • Y5 (Ex. BP620/60, DM 660, Em.  BP700/75)
  • Objective lenses
    • HC PL FLUOTAR 20x/0.50
    • HC PL APO 63x/1.40­0.60 OIL
    • HCX PL APO 40x/1.30 OIL
    • HCX PL APO 100x/1.40­0.70 OIL
  • Black/White CCD camera with NIR mode (Leica DFC365 FX)
    • max 21 fps
    • 1392x1040 pixels
  • LAS X software

Contact us for more information

Location

Faculty Bioscience Engineering,

Vakgroep Biotechnologie, BW25

Campus Proeftuin, Gebouw N1, Lokaal 36.01.100.030

B-9000, Ghent

Price

Price category 1

Lionheart™ FX Automated Microscope 

Optimized for live cell imaging with brightfield, color brightfield and fluorescence channels.

  • Sampleholder 
    • four 35 mm round dishes
    • multiwell plates
    • 2-position slide holder
  • Environmental control cover: For temperature control to 40 °C and "darkroom" fluorescence, incl. Humidity control chamber
  • Objectives
    • 20x Objective, Olympus Plan Apochromat, NA 0.4, WD 1.2
    • 2.5x (2.75x eff), Zeiss Plan Fluorite, NA 0.12, WD 2.5
    • High contrast brightfield kit, 4x Objective included
  • Filter cubes & LEDs
    • DAPI filter cube: Ex. 377/50 Em. 447/60 mirror 409
      365 nm LED cube
    • GFP filter cube: Ex. 469/35 Em. 525/39 mirror 497
      465 nm LED cub
    • Texas Red filter cube: Ex. 586/15 Em. 647/57 mirror 605
      590 nm LED cube
    • CY5 Filter cube: Ex. 628/40 Em. 685/40 mirror 660
      623nm LED cube
  • Software GEN5IPRIME (Gen5 Image Prime)

Contact us for more information

Location

Faculty of Medicine and Health Sciences,

Department of Basic Medical Sciences,

C. Heymanslaan 10, Blok B,

B-9000, Ghent

Price

Price category 1