Program:

• 12.00 – 12.05: Welcome by Prof. Ruslan Dmitriev
• 12.05 – 12.10: Introduction to GLiM by dr. Herlinde De Keersmaecker
• 12.10 – 13.00:
  • Introduction to the STELLARIS FALCON by Prof. Ruslan Dmitriev (UGent)
  • Application lecture by Dr. Luis Alvarez, (Leica Mannheim)
• 13.00 – 14.00: Sandwich lunch with the possibility to take a look at the microscope (10 min)
• 14.00 – 17.00: '1 hour' demonstrations

Register here before 5 December 2022

What is Fluorescence Lifetime Imaging Microscopy?

In fluorescence lifetime imaging microscopy (FLIM) the fluorescence lifetime is used instead of the spectral  property of fluorescence. Fluorescence lifetime is a measure of how long a fluorescent molecule or fluorophore remains in its excited state before returning to the ground state by emitting a fluorescence photon. The decay of a fluorophore does not always occurs at exact the same time after excitation but a distribution of decay times is observed resulting in a characteristic time constant. This decay is dependent on the fluorophore and is highly sensitivity to the molecular environment and changes in molecular conformation. Flim can therefore be used as an alternative to separate fluorophores, to study cellular metabolism using autofluorescent molecular imaging, to monitor microenvironmental parameters (such as temperature, viscosity, pH, and ion concentration), using FLIM-based sensors and to study protein–protein interactions using Förster resonance energy transfer (FRET) sensors.